Effects of antimicrobial peptide and tributyrin on fecal microflora and blood indices of female calves

This study evaluated the effects of antimicrobial peptide (AMP) and tributyrin (TB) on dairy calves in terms growth performance, immunity, oxidative stress, intestinal microflora. A total 40 female were divided into four treatment groups (n = 10): basal diet +0.015% essential oil, +0.03% AMP, +0.15% TB, AMP + 0.15% TB. TB supplementation increased average daily gain (ADG) weaning weight, while reducing diarrhea occurrence. Additionally, reduced levels reactive oxygen species (ROS) malonaldehyde (MDA), increasing superoxide dismutase (SOD) serum immunoglobulin M (IgM) levels. However, combined use did not significantly affect feed intake, ADG, or incidence but decreased ROS levels, SOD as well MDA IgM Moreover, TG relative abundance several beneficial fiber- mucin-degrading bacteria gut, contrast to supplementation. The 16S rRNA results showed that Rikenellaceae_RC9_gut_group, Ruminococcaceae_UCG-014 [Eubacterium]_coprostanoligenes group (p < .01), Ruminococcaceae_UCG-005 Christensenellaceae_R-7_group .01). abundances Rikenellaceae_RC9_gut_group Ruminococcaceae_UCG-014, Bacteroides In summary, diets supplemented with either improved microflora, health weaned calves, was detrimental calf performance.